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Research & Validation Reports

Better Biosecurity has developed a proprietary micro-spray system for applying a safe, non-toxic, and organic disinfectant (Paerolyte) within a livestock facility to eliminate viruses and bacteria. Paerolyte (formerly known as Paerosol) is an EPA-registered medical grade disinfectant which has repeatedly demonstrated up to 6-log reductions (99.9999%) in dozens of pathogens including Influenza A (H5N1), H1N1(Swine Flu), MG, MRSA, PRRSV, E. Coli, C. Diff, Salmonella, Pseudomonas and African Swine Fever Virus.

Better Biosecurity’s micro-spray system builds on technology originally developed by the U. S. Department of Energy to safeguard humans from real-time bioterrorism threats. Paerosol has been validated by government agencies and leading research institutions including the U.S. Department of Energy, U.S. Department of Agriculture, U.S. Defense Threat Reduction Agency (DTRA), Auburn University, University of Minnesota, and the University of Colorado to eliminate bacteria, viruses, and spores both in the air and on surfaces. 

Full Research Papers Available Upon Request

Auburn University Egg Holding and Incubation Study with Paerosol Dry Spray Technology

Conclusion:  Paerolyte Micro-Spray was proven effective at lowering mortality, increasing birth weight and 7-day growth rates in a hatchery facility in repeated experiments.

By:  Dr. Wallace Berry Ph. D., Dr. Ken Macklin Ph. D.

U.S. Department of Agriculture

Mississippi State University

Application of a Micro-Aerosolized Disinfectant to clear Mycoplasma Gallisepticum (MG) from Contaminated Facilities

Conclusion:  The Paerolyte solution was effective against actively growing cells of the avian pathogen MG and was effective independent of MG location (wall or ceiling) and position (vertical or inverted) in the chamber indicating the ability of the micro-sprayer to saturate the environment inside the enclosed facility with small disinfecting particles resistant to gravitational effects.

By:   J. D. Evans, S. L. Branton, S. D. Collier, J. P. Brooks, and J. L. Purswell
USDA-Agricultural Research Service, Mississippi State, MS

U.S. Department of Agriculture

Mississippi State University

Application of a Micro-Aerosolized Disinfectant to clear Mycoplasma Gallisepticum (MG) from Contaminated Facilities

Conclusion:  The Paerolyte solution was effective against actively growing cells of the avian pathogen MG and was effective independent of MG location (wall or ceiling) and position (vertical or inverted) in the chamber indicating the ability of the micro-sprayer to saturate the environment inside the enclosed facility with small disinfecting particles resistant to gravitational effects.

By:   J. D. Evans, S. L. Branton, S. D. Collier, J. P. Brooks, and J. L. Purswell
USDA-Agricultural Research Service, Mississippi State, MS

Toxicity study of Paerosol in the conditions simulating it application for volumetric disinfection of enclosed environment

Conclusion:  The results of the study demonstrated that animal behavior and general conditions (skin and hair) were unchanged. Macroscopic and microscopic examination of spleen, liver, and lung tissues revealed no pathology.  No toxic effect of PAEROSOL micro-spray toward the immune system of mice was revealed even up to 20 hours of exposure.

By:  E. Rainina, Principal Investigator, Pacific Northwest National Laboratory

Paerosol efficacy toward airborne and surfaced H5N1 viruses

Conclusion:   Airborne Influenza A (H5N1) virus infectivity was reduced below detection level during 4 minutes of exposure in each of 3 conducted experiments.

By:  E. Rainina, Principal Investigator, Pacific Northwest National Laboratory

Toxicity study of Paerosol in the conditions simulating it application for volumetric disinfection of enclosed environment

Conclusion:  The results of the study demonstrated that animal behavior and general conditions (skin and hair) were unchanged. Macroscopic and microscopic examination of spleen, liver, and lung tissues revealed no pathology.  No toxic effect of PAEROSOL micro-spray toward the immune system of mice was revealed even up to 20 hours of exposure.

By:  E. Rainina, Principal Investigator, Pacific Northwest National Laboratory

Paerosol efficacy toward airborne and surfaced H5N1 viruses

Conclusion:   Airborne Influenza A (H5N1) virus infectivity was reduced below detection level during 4 minutes of exposure in each of 3 conducted experiments.

By:  E. Rainina, Principal Investigator, Pacific Northwest National Laboratory

University of Colorado Boulder

Summary of the Paerolyte Bioaerosol Disinfection Trials

Conclusion:  Paerolyte Micro-Spray was proven effective at reducing the presence of airborne C. Diff (Bacillus subtilis spores used as a surrogate) by a minimum of 99.99%.

By: Dr. Mark Hernandez, Professor of Civil and Environmental Engineering
Environmental Engineering Microbiology & Disinfection Laboratory

University of Minnesota

Application of the RISNA qPCR module to determine the efficacy of Hypochlorous Acid (HOCl), as supplied by Paerosol, to inactivate the African Swine Fever megavirus

Conclusion:  No presence of megaviral ASFV DNA (Evh Surrogate) in liquid treated with the Paerolyte at 500 ppm for up to 1 minute.

By:  Dr Declan Schroeder, Associate Professor of Virology
College of Veterinary Medicine

Application of the V-qPCR assay to determine the efficacy of Paerolyte to inactivate PRRSV

Conclusion:  No presence of PRRSV RNA in liquid treated with Paerolyte  at 400ppm for up to 1 minute.

By:  Dr Declan Schroeder, Associate Professor of Virology
College of Veterinary Medicine

University of Minnesota

Application of the RISNA qPCR module to determine the efficacy of Hypochlorous Acid (HOCl), as supplied by Paerosol, to inactivate the African Swine Fever megavirus

Conclusion:  No presence of megaviral ASFV DNA (Evh Surrogate) in liquid treated with the Paerolyte at 500 ppm for up to 1 minute.

By:  Dr Declan Schroeder, Associate Professor of Virology
College of Veterinary Medicine

Application of the V-qPCR assay to determine the efficacy of Paerolyte to inactivate PRRSV

Conclusion:  No presence of PRRSV RNA in liquid treated with Paerolyte  at 400ppm for up to 1 minute.

By:  Dr Declan Schroeder, Associate Professor of Virology
College of Veterinary Medicine

U.S. Defense Threat Reduction Agency

Exploratory Use of Micro-Aerosol Decontamination Technology (PAEROSOL) in Enclosed, Unoccupied Hospital Setting

Conclusion:   Paerosol micro-spray was shown capable of eradicating 106/cm2 live microorganisms, including Gram- positive and Gram-negative bacteria, microbial and fungal spores, and viruses on various materials and surfaces, including the surfaces of complex geometry. The array of examined bioagents includes simulates of bio-threat agents (Anthrax simulate), civilian and military hospitals pathogens, antibiotic-resistant microbes, viruses including H1N1 (swine flu), and H5N1 (bird flu) subtypes, as well as food-, and water-borne pathogens.

By:  E. Rainina, Principal Investigator, Pacific Northwest National Laboratory

United States Department of Agriculture
Agricultural Marketing Service

National Organic Program Policy Memorandum (PM 15-4)

Conclusion:  NOP determines and clarifies that Hypochlorous Acid generated from electrolyzed water is allowable for organic food production and handling.

United States Department of Agriculture
Agricultural Marketing Service

National Organic Program Policy Memorandum (PM 15-4)

Conclusion:  NOP determines and clarifies that Hypochlorous Acid generated from electrolyzed water is allowable for organic food production and handling.